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| Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko. |
| An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020 |
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| Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko. |
| In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025 |
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| Ono,Elisabete Yurie Sataque; Biazon,Luciana; Silva,Marcelo da; Vizoni,Édio; Sugiura,Yoshitsugu; Ueno,Yoshio; Hirooka,Elisa Yoko. |
| Natural fungal and fumonisin contamination were evaluated in 109 freshly harvested corn samples from Paraná State and correlated to damaged kernels (%). In addition, healthy and damaged kernels of 24 corn samples were selected in order to compare the mycoflora profile and fumonisin levels. The correlation among protein/lipid content and fumonisin levels was also analyzed in the 15 most frequently cultivated corn hybrids. Total fungal colony count in 109 freshly harvested corn samples ranged from 1.9x10(4) to 3.5x10(6) CFU/g, Fusarium sp. count from 1.0x10³ to 2.2x10(6) CFU/g, and fumonisin levels from 0.13 to 20.38 µg/g. Total fungal colony/Fusarium sp. count and fumonisin levels showed positive correlation (p < 0.05). In addition, there was a positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoflora; Toxigenic fungi; Mycotoxins; Fumonisins; Corn quality. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100008 |
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| Ono,Elisabete Yurie Sataque; Fungaro,Maria Helena Pelegrinelli; Sofia,Silvia Helena; Miguel,Tatiana de Ávila; Sugiura,Yoshitsugu; Hirooka,Elisa Yoko. |
| In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 µg/g, and from 1.18 to 1209.91 µg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 µg/g), eight as intermediate (>1289.84 to 3772.44 µg/g) and four strains as high (>3772.44 µg/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fumonisin; Fusarium verticillioides; Genetic variability; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400026 |
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| Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli. |
| Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009 |
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| Coelho,Alexandre Rodrigo; Celli,Marcos Giovani; Ono,Elisabete Yurie Sataque; Wosiacki,Gilvan; Hoffmann,Fernando Leite; Pagnocca,Fernando Carlos; Hirooka,Elisa Yoko. |
| Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased the initial patulin concentration of 588.4 to 290.0 µg/mL, ability of P. ohmeri 158 in biocontrol against Penicillium expansum and patulin decrease in vitro was performed. The culture supernatant of P. ohmeri 158 was effective against 66.17% micelial growth, indicating antibiosis related with the killer phenomenon. The initial patulin concentration of 223 µg in the presence of P. ohmeri 158 cells was decreased over 83% of the original concentration, when incubated at 25ºC/2 days and > 99% after 5 days incubation time, with undetectable patulin level after 15 days. The initial pH 4.0 decreased to pH 3.3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillium expansum; Antagonist yeasts; Biocontrol; Patulin; Degradation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400019 |
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